Journal: APL Bioengineering

Article Title: Skin wound healing improvement in diabetic mice through FTIR microspectroscopy after implanting pluripotent stem cells

doi: 10.1063/5.0130383

Figure Lengend Snippet: FTIR microspectroscopy (FTIRM) spectra. (a) FTIRM spectra of the epidermis at day 7 post-skin lesion excision in the ISS and PSCs groups. It can be observed that the bands related to lipids, collagen, phospholipids, and nuclei acids showed a higher absorbance in the PSCs group. (b) FTIRM spectra of the epidermis at day 15 post-skin lesion excision in the ISS and PSCs groups. It is shown that the bands related to amide I and amide II had a higher absorbance in the PSCs group. (c) FTIRM spectra of the dermis at day 7 post-skin lesion excision in the ISS and PSCs groups. It is evidenced that the bands related to amide I, amide II, lipids, and phospholipids depicted a higher absorbance in the PSCs group. (d) FTIRM spectra of the dermis at day 15 post-skin lesion excision in the ISS and PSCs groups. It is shown that the bands related to amide I, amide II, lipids, and collagen had a higher absorbance in the ISS group.

Article Snippet: After that, the samples were analyzed three times employing a Cassegrain objective of 16× of an FTIR microscope (Jasco; IRT-5200) coupled to an FTIR spectrometer (Jasco; 6600).

Techniques:

Journal: APL Bioengineering

Article Title: Skin wound healing improvement in diabetic mice through FTIR microspectroscopy after implanting pluripotent stem cells

doi: 10.1063/5.0130383

Figure Lengend Snippet: Micro-FTIR images (FTIRI) of the skin samples of the ISS and PSCs groups at 7 and 15 days post-skin lesion excision, showing the biochemical map of different biomolecules and their biodistribution. Epidermis (1) and dermis (2) can be distinguished. The lipids analysis (1737 cm −1 ) demonstrated a higher amount of this molecule in the PSCs group than in the ISS group. In contrary, the ceramides of amide I (1666 cm −1 ) and ceramides of amide II (1549 cm −1 ) analysis showed a great amount of these biomolecules in the ISS group at day 7, highlighting that in the collagen (1400 cm −1 ) and collagen triple helix (1660 cm −1 ) analysis the concentration of these molecules diminished over time in the ISS group. However, these molecules showed a greater amount in the PSCs group than in the ISS group. Finally, the analysis of the disordered structure of proteins (1638 cm −1 ) and collagen fibers aligned (amide I/amide II) showed that the ISS group evidenced a major content. Red and blue colors represent the strong and weak absorption of the infrared beam.

Article Snippet: After that, the samples were analyzed three times employing a Cassegrain objective of 16× of an FTIR microscope (Jasco; IRT-5200) coupled to an FTIR spectrometer (Jasco; 6600).

Techniques: Concentration Assay

Journal: Optics Express

Article Title: Gigahertz semiconductor laser at a center wavelength of 2 µm in single and dual-comb operation

doi: 10.1364/oe.503035

Figure Lengend Snippet: Fig. 5. Heterodyne beat of the dual-comb outputs recorded on a fast photodiode. Time traces undergo digital filtering using a 500 MHz low-pass filter. a) Single interferogram resulting from the beat of the two pulse trains. b) Zoomed-out view of the interferogram traces. c) Fast Fourier transform (FT) of a single interferogram, revealing the microwave spectrum generated by the beat of the two combs. d) FT of the interferogram traces resulting into the microwave comb. The inset provides a magnified view of the individual comb lines. e) Evolution of the first and second-order differences in the interferogram phase over the entire 200 ms recording time. Notably, the second-order difference of the phase remains bounded between ± π.

Article Snippet: The heterodyne beat note is analyzed using the same fast photodiode connected to a Teledyne LeCroy WAVEPRO 254HD oscilloscope (5 GHz), employing a 2.85 GHz low pass filter (Mini Circuits VLF-2850+).

Techniques: Generated